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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Microalbunminuria ELISA kit

E02M0022 Rat Microalbunminuria ELISA kit

The Rat Microalbunminuria ELISA kit can be used to identify samples from the rat species. Microalbunminuria  can also be called MAU.

Specifications of Rat Microalbunminuria ELISA kit

Product Information

Cat. No.

E02M0022

Product Name

Rat Microalbunminuria ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 mg/L

Sensitivity

1.0 mg/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/L

1 vial

STANDARD B (0.5mL)

5.0 mg/L

1 vial

STANDARD C (0.5mL)

10 mg/L

1 vial

STANDARD D (0.5mL)

25 mg/L

1 vial

STANDARD E (0.5mL)

50 mg/L

1 vial

STANDARD F (0.5mL)

100 mg/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MAU ELISA kit uses an anti-IMAU antibody and an IMAU-HRP conjugate in a competitive enzyme immunoassay method. IMAU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IMAU antibody binding site between IMAU from samples and IMAU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IMAU. Since the number of sites is limited, as more sites are occupied by IMAU from the sample, fewer sites are left to bind IMAU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMAU concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Microalbunminuria ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MAU and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Microalbunminuria ELISA kit

Summary of the Assay Procedure for Rat Microalbunminuria ELISA kit

Citations of Rat Microalbunminuria ELISA kit

E02M0022 has been referenced in the below publications:

EXPERIMENTAL STUDY OF HAIKUNSHENXI CAPSULE AND BLILING CAPSULE ON HYPERURICEMIC NEPHROPATHY.

Effect of Chinese Herbs for Stasis Removing and Collaterals Dredging upon Angiotensin-Converting Enzyme 2-Angiotensin-(1-7)Mas Axis in the Renal Cortex of Diabetic Nephropathy Rats.

EXPERIMENTAL STUDY OF HAIKUNSHENXI CAPSULE AND BLILING CAPSULE ON HYPERURICEMIC NEPHROPATHY.

EFFECT OF HAIKUNSHENXI CAPSULE ON HYPERURICEMIC NEPHROPATHY.


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