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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E02R0005 Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

The Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit can be used to identify samples from the rat species. Receptor Activator of Nuclear Factor Kb Ligand can also be called CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine, RANKL.

Specifications of Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Product Information

Cat. No.

E02R0005

Product Name

Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RANKL ELISA kit uses an anti-RANKL antibody and an RANKL-HRP conjugate in a competitive enzyme immunoassay method. RANKL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RANKL antibody binding site between RANKL from samples and RANKL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RANKL. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RANKL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Summary of the Assay Procedure for Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Citations of Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E02R0005 has been referenced in the below publications:

Effects of combination of Tripterygium Glycoside with Ginsenoside on the proliferation and RANKL/OPG expression of rat fibroblast-like synoviocytes induced by Macrophage migration inhibitory factor.

The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.


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