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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Secretory Immunoglobulin A ELISA kit

E02S0003 Rat Secretory Immunoglobulin A ELISA kit

The Rat Secretory Immunoglobulin A ELISA kit can be used to identify samples from the rat species. Secretory Immunoglobulin A can also be called SIgA, secretory IgA.

Specifications of Rat Secretory Immunoglobulin A ELISA kit

Product Information

Cat. No.

E02S0003

Product Name

Rat Secretory Immunoglobulin A ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SIgA ELISA kit uses an anti-SIgA antibody and an SIgA-HRP conjugate in a competitive enzyme immunoassay method. SIgA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SIgA antibody binding site between SIgA from samples and SIgA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SIgA. Since the number of sites is limited, as more sites are occupied by SIgA from the sample, fewer sites are left to bind SIgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIgA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Secretory Immunoglobulin A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SIgA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Secretory Immunoglobulin A ELISA kit

Summary of the Assay Procedure for Rat Secretory Immunoglobulin A ELISA kit

Citations of Rat Secretory Immunoglobulin A ELISA kit

E02S0003 has been referenced in the below publications:

Protective Effects and Mechanism of Erythropoietin on Necrotizing Enterocolitis in a Neonatal Rat Model.

The protective effect and mechanism of alanyl-glutamine dipeptide in treatment of severe acute pancreatitis.

The expression variety of slaA and secretory component in small intestinal tract under hvperoxia environment.

The effects of TP-5 resuscitating serious traumatic hemorrhagic shock on immune function in rats.

Effects of colon targeting microecological modulator nano CYP synbiotics on cytokines and expression of SOD,MDA, NO and MPO in dysbacteria wistar rats.

Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.

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