E02S0003 Rat Secretory Immunoglobulin A ELISA kit
The Rat Secretory Immunoglobulin A ELISA kit can be used to identify samples from the rat species. Secretory Immunoglobulin A can also be called SIgA, secretory IgA.
Specifications of Rat Secretory Immunoglobulin A ELISA kit
Product Information | |
Cat. No. | E02S0003 |
Product Name | Rat Secretory Immunoglobulin A ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SIgA ELISA kit uses an anti-SIgA antibody and an SIgA-HRP conjugate in a competitive enzyme immunoassay method. SIgA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SIgA antibody binding site between SIgA from samples and SIgA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SIgA. Since the number of sites is limited, as more sites are occupied by SIgA from the sample, fewer sites are left to bind SIgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIgA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Secretory Immunoglobulin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SIgA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Secretory Immunoglobulin A ELISA kit
Citations of Rat Secretory Immunoglobulin A ELISA kit
E02S0003 has been referenced in the below publications：
Protective Effects and Mechanism of Erythropoietin on Necrotizing Enterocolitis in a Neonatal Rat Model.
The protective effect and mechanism of alanyl-glutamine dipeptide in treatment of severe acute pancreatitis.
The expression variety of slaA and secretory component in small intestinal tract under hvperoxia environment.
The effects of TP-5 resuscitating serious traumatic hemorrhagic shock on immune function in rats.
Effects of colon targeting microecological modulator nano CYP synbiotics on cytokines and expression of SOD,MDA, NO and MPO in dysbacteria wistar rats.
Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
