E02E0167 Rat Epiligrin ELISA kit
The Rat Epiligrin ELISA kit can be used to identify samples from the rat species. Epiligrin can also be called EP.
Specifications of Rat Epiligrin ELISA kit
Product Information | |
Cat. No. | E02E0167 |
Product Name | Rat Epiligrin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EP ELISA kit uses an anti-EP antibody and an EP-HRP conjugate in a competitive enzyme immunoassay method. EP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EP antibody binding site between EP from samples and EP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EP. Since the number of sites is limited, as more sites are occupied by EP from the sample, fewer sites are left to bind EP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Epiligrin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Epiligrin ELISA kit
Citations of Rat Epiligrin ELISA kit
E02E0167 has been referenced in the below publications：
The Study on the Expression of Extrasynaptic GABAA Receptors in Specific Brain Regions during Different Phase of Estrus Cycle in Rodents.
