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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Epiligrin ELISA kit

E02E0167 Rat Epiligrin ELISA kit

The Rat Epiligrin ELISA kit can be used to identify samples from the rat species. Epiligrin can also be called EP.

Specifications of Rat Epiligrin ELISA kit

Product Information

Cat. No.

E02E0167

Product Name

Rat Epiligrin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EP ELISA kit uses an anti-EP antibody and an EP-HRP conjugate in a competitive enzyme immunoassay method. EP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EP antibody binding site between EP from samples and EP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EP. Since the number of sites is limited, as more sites are occupied by EP from the sample, fewer sites are left to bind EP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Epiligrin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Epiligrin ELISA kit

Summary of the Assay Procedure for Rat Epiligrin ELISA kit

Citations of Rat Epiligrin ELISA kit

E02E0167 has been referenced in the below publications:

The Study on the Expression of Extrasynaptic GABAA Receptors in Specific Brain Regions during Different Phase of Estrus Cycle in Rodents.


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