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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Alpha Smooth Muscle Actin ELISA kit

E02S0004 Rat Alpha Smooth Muscle Actin ELISA kit

The Rat Alpha Smooth Muscle Actin ELISA kit can be used to identify samples from the rat species. Alpha Smooth Muscle Actin can also be called AAT6, ACTSA, MYMY5, Alpha  SMA.

Specifications of Rat Alpha Smooth Muscle Actin ELISA kit

Product Information

Cat. No.

E02S0004

Product Name

Rat Alpha Smooth Muscle Actin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

50 ng/ml

1 vial

STANDARD C (0.5mL)

100 ng/ml

1 vial

STANDARD D (0.5mL)

250 ng/ml

1 vial

STANDARD E (0.5mL)

500 ng/ml

1 vial

STANDARD F (0.5mL)

1000 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Alpha  SMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Alpha  SMA. Standards or samples are then added to the microtiter plate wells and Alpha  SMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Alpha  SMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Alpha  SMA are added to each well to “sandwich” the Alpha  SMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Alpha  SMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha  SMA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Alpha Smooth Muscle Actin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Alpha  SMA and analogues was observed.


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Summary of the Assay Procedure for Rat Alpha Smooth Muscle Actin ELISA kit

Summary of the Assay Procedure for Rat Alpha Smooth Muscle Actin ELISA kit

Citations of Rat Alpha Smooth Muscle Actin ELISA kit

E02S0004 has been referenced in the below publications:

Discussion of changes of TGF-β 1,Smad-4,α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the“liver and kidney share the same origin”.


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