E02I0011 Rat Inositol Triphosphate ELISA kit
The Rat Inositol Triphosphate ELISA kit can be used to identify samples from the rat species. Inositol Triphosphate can also be called InsP3, Inositol 1,4,5-Trisphosphate, Triphosphoinositol, IP3.
Specifications of Rat Inositol Triphosphate ELISA kit
Product Information | |
Cat. No. | E02I0011 |
Product Name | Rat Inositol Triphosphate ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IP3 ELISA kit uses an anti-IP3 antibody and an IP3-HRP conjugate in a competitive enzyme immunoassay method. IP3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IP3 antibody binding site between IP3 from samples and IP3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IP3. Since the number of sites is limited, as more sites are occupied by IP3 from the sample, fewer sites are left to bind IP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Inositol Triphosphate ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IP3 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Inositol Triphosphate ELISA kit
Citations of Rat Inositol Triphosphate ELISA kit
E02I0011 has been referenced in the below publications：
Juvenile hormone-activated phospholipase C pathway enhances transcriptional activation by the methoprene-tolerant protein.
Effect of Regulating Liver and Invigorating Spleen Decoction on the function of antral mucosa and smooth muscle in rats with functional dyspepsia ( syndrome of liver-qi stagnation and spleen-qi deficidency ).
