E02F0004 Rat Fibronectin ELISA kit
The Rat Fibronectin ELISA kit can be used to identify samples from the rat species. Fibronectin can also be called CIG, Cold insoluble globulin, ED B, ED-B, fibronectin 1, FINC, FN 1 FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Transformation sensitive protein, Fibronectin, Cold-insoluble globulin.
Product Information | |
Cat. No. | E02F0004 |
Product Name | Rat Fibronectin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FN ELISA kit uses an anti-FN antibody and an FN-HRP conjugate in a competitive enzyme immunoassay method. FN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FN antibody binding site between FN from samples and FN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FN. Since the number of sites is limited, as more sites are occupied by FN from the sample, fewer sites are left to bind FN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FN concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FN and analogues was observed. | |
E02F0004 has been referenced in the below publications：
Effects of Sparganii Pill Treating Intestinal Fibrosis in Rats of Crohn's Disease.
Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats.
Curcumin Suppresses Intestinal Fibrosis by Inhibition of PPARr-Mediated Epithelial-Mesenchymal Transition.
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