E02N0008 Rat N terminal pro brain natriuretic peptide ELISA kit
The Rat N terminal pro brain natriuretic peptide ELISA kit can be used to identify samples from the rat species. N terminal pro brain natriuretic peptide can also be called BNPT, NT proBNP.
Product Information | |
Cat. No. | E02N0008 |
Product Name | Rat N terminal pro brain natriuretic peptide ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NT proBNP ELISA kit uses an anti-NT proBNP antibody and an NT proBNP-HRP conjugate in a competitive enzyme immunoassay method. NT proBNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NT proBNP antibody binding site between NT proBNP from samples and NT proBNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NT proBNP. Since the number of sites is limited, as more sites are occupied by NT proBNP from the sample, fewer sites are left to bind NT proBNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT proBNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NT proBNP and analogues was observed. | |
E02N0008 has been referenced in the below publications：
Effects of fasudil on pulmonary arterial hypertension with vascular remodeling in rats.
Effects of exercise traning on ventricular remodeling following myocardial infarction in rats.
The influence of recombinant human brain natriuretic peptide and furosemide on diuretic effect in rats with heart failure with reduced ejection fraction and its mechanism.
Sacubitril/Valsartan Decreases Atrial Fibrillation Susceptibility by Inhibiting Angiotensin II-Induced Atrial Fibrosis Through p-Smad2/3, p-JNK, and p-p38 Signaling Pathways.
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