E02P0059 Rat Pappalysin 2 ELISA kit
The Rat Pappalysin 2 ELISA kit can be used to identify samples from the rat species. Pappalysin 2 can also be called PAPP-A2, PAPPE, PLAC3, Placenta-Specific 3, Pregnancy-associated plasma protein A2, Pregnancy-associated plasma protein E1, PAPPA2.
Product Information | |
Cat. No. | E02P0059 |
Product Name | Rat Pappalysin 2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 25 pg/ml | 1 vial |
STANDARD C (0.5mL) | 50 pg/ml | 1 vial |
STANDARD D (0.5mL) | 100 pg/ml | 1 vial |
STANDARD E (0.5mL) | 250 pg/ml | 1 vial |
STANDARD F (0.5mL) | 500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PAPPA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAPPA2. Standards or samples are then added to the microtiter plate wells and PAPPA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAPPA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PAPPA2 are added to each well to “sandwich” the PAPPA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAPPA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAPPA2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PAPPA2 and analogues was observed. | |
E02P0059 has been referenced in the below publications：
Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific.
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