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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Follicle Stimulating Hormone ELISA kit

E02F0001 Rat Follicle Stimulating Hormone ELISA kit

The Rat Follicle Stimulating Hormone ELISA kit can be used to identify samples from the rat species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta, FSH.

Specifications of Rat Follicle Stimulating Hormone ELISA kit

Product Information

Cat. No.

E02F0001

Product Name

Rat Follicle Stimulating Hormone ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

5.0 ng/ml

1 vial

STANDARD C (0.5mL)

10 ng/ml

1 vial

STANDARD D (0.5mL)

25 ng/ml

1 vial

STANDARD E (0.5mL)

50 ng/ml

1 vial

STANDARD F (0.5mL)

100 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FSH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FSH. Standards or samples are then added to the microtiter plate wells and FSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FSH are added to each well to “sandwich” the FSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Follicle Stimulating Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FSH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Follicle Stimulating Hormone ELISA kit

Summary of the Assay Procedure for Rat Follicle Stimulating Hormone ELISA kit

Citations of Rat Follicle Stimulating Hormone ELISA kit

E02F0001 has been referenced in the below publications:

The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.

Influences of Maca and Tongkat Ali on The Serum Testosterone Level of Swimming Training Rats.

以肝脾为核也干预HPRL模型大鼠下丘脑多己胺腺昔酸环化酶通路机制的探索。

EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.

The study on the effects of quercetin,vitamin E and amifostine on Cisplatin-induced ovarian damage in famale rats.

东革阿里对游泳训练大鼠血睾酮水平及运动能力的影响。

Effects of the timing of estrogen replacement therapy on cognitive function of ovariectomized rats.

Effect of Melatonin Treatment during Pregnancy on Growth of Female Offspring in Rat.

参麦黄连阿胶汤治疗绝经综合征的药效学实验。

Quantitative changes in testicular structure and function in rat exposed to mobile phone radiation.


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