E02A0203 Rat Acetylcholine Receptor antibody ELISA kit
The Rat Acetylcholine Receptor antibody ELISA kit can be used to identify samples from the rat species. Acetylcholine Receptor antibody can also be called cholinergic receptors;muscarinic acetylcholine receptor, M-AChR M1, ACHRAB.
Product Information | |
Cat. No. | E02A0203 |
Product Name | Rat Acetylcholine Receptor antibody ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ACHRAB ELISA kit uses an anti-ACHRAB antibody and an ACHRAB-HRP conjugate in a competitive enzyme immunoassay method. ACHRAB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACHRAB antibody binding site between ACHRAB from samples and ACHRAB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACHRAB. Since the number of sites is limited, as more sites are occupied by ACHRAB from the sample, fewer sites are left to bind ACHRAB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACHRAB concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ACHRAB and analogues was observed. | |
E02A0203 has been referenced in the below publications：
Effect of Bupi Qiangli Compound on mRNA Expression Level of Glucocorticoid Receptor in Thymus of EAMG Model Rats.
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