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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat 5 Hydroxyindoleacetic Acid ELISA kit

  • Epigenetics and Nuclear Signaling

E02H0320 Rat 5 Hydroxyindoleacetic Acid ELISA kit

The Rat 5 Hydroxyindoleacetic Acid ELISA kit can be used to identify samples from the rat species. 5 Hydroxyindoleacetic Acid can also be called (5-Hydroxy-1H-indol-3-yl)acetic acid, 5 HIAA.

Specifications of Rat 5 Hydroxyindoleacetic Acid ELISA kit

Product Information

Cat. No.

E02H0320

Product Name

Rat 5 Hydroxyindoleacetic Acid ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

5 HIAA ELISA kit uses an anti-5 HIAA antibody and an 5 HIAA-HRP conjugate in a competitive enzyme immunoassay method. 5 HIAA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-5 HIAA antibody binding site between 5 HIAA from samples and 5 HIAA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 5 HIAA. Since the number of sites is limited, as more sites are occupied by 5 HIAA from the sample, fewer sites are left to bind 5 HIAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HIAA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 5 Hydroxyindoleacetic Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 5 HIAA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 5 Hydroxyindoleacetic Acid ELISA kit

Summary of the Assay Procedure for Rat 5 Hydroxyindoleacetic Acid ELISA kit

Citations of Rat 5 Hydroxyindoleacetic Acid ELISA kit

E02H0320 has been referenced in the below publications:

参麦黄连阿胶汤治疗绝经综合征的药效学实验。

Multi-omic Profiling Reveals that Intra-abdominal Hypertension-Induced Intestinal Damage Can Be Prevented by Microbiome and Metabolic Modulations with 5-Hydroxyindoleacetic Acid as a Diagnostic Marker.

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