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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Tumor Necrosis Factor Alpha ELISA kit (E02T0008)

E02T0008 Rat Tumor Necrosis Factor Alpha ELISA kit

The Rat Tumor Necrosis Factor Alpha ELISA kit  can be used to identify samples from the rat species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2.

Products

Specifications of Rat Tumor Necrosis Factor Alpha ELISA kit

Product Information

Cat. No.

E02T0008

Product Name

Rat Tumor Necrosis Factor Alpha ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNF-α ELISA kit uses an anti-TNF-α antibody and an TNF-α-HRP conjugate in a competitive enzyme immunoassay method. TNF-α-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNF-α antibody binding site between TNF-α from samples and TNF-α-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNF-α. Since the number of sites is limited, as more sites are occupied by TNF-α from the sample, fewer sites are left to bind TNF-α-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF-α concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Tumor Necrosis Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNF-α and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Tumor Necrosis Factor Alpha ELISA kit

Summary of the Assay Procedure for Rat Tumor Necrosis Factor Alpha ELISA kit

Citations of Rat Tumor Necrosis Factor Alpha ELISA kit

E02T0008 has been referenced in the below publications:

Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model.

Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.

Oxymatrine Improves Intestinal Epithelial Barrier Function Involving NF-kB-Mediated Signaling Pathway in CCl4-Induced Cirrhotic Rats.

Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.

The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells.

Atrial Fibrillation Promotion in a Rat Model of Rheumatoid Arthritis.

The Mechanisms Underlying the Antidepressant Effect of Acupuncture via Regulating the Immune/Inflammatory Response Mediated by TLR4 Signaling Pathway/NLRP3 Inflammasome in Rats Exposed to Chronic Restraint Stress.

The research of the role of the p38MAPK in the acute lung injury induced by PO in rats.

Progesterone protects neurons against impairment induced by Aβ activated astrocytes.

Rat Interleukin 6 ELISA kit; Rat Interleukin 10 ELISA kit; Rat Connective Tissue Growth Factor ELISA kit; Rat Cyclooxygenase 2 ELISA kit; Rat Immunoglobulin G ELISA kit; Rat Prostaglandin E2 ELISA kit; Rat Tumor Necrosis Factor Alpha ELISA kit; Rat Transforming Growth Factor β ELISA kit; Rat Rheumatoid Factor ELISA kit.

THE STUDY OF FASUDIL HYDROCHLORIDE PREVENTED ACUTE KIDNEY INJURY RAT CAUSED BY LIPOPOLYSACCHARIDE.

Effects of Qingchang Wenzhong Decoction on intestinal mucosa barrier in colitis rats induced by TNBS.

A Modified Passive Smoking and Endo-tracheal Instillation of LPS Method for Establishment of COPD Models in Rats.

Untargeted Metabolomic Analysis of the Effects and Mechanism of Nuciferine Treatment on Rats With Nonalcoholic Fatty Liver Disease.

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