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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Deoxycholic Acid ELISA kit

E02D0314 Rat Deoxycholic Acid ELISA kit

The Rat Deoxycholic Acid ELISA kit can be used to identify samples from the rat species. Deoxycholic Acid can also be called Deoxycholate, cholanoic acid, Kybella, DCA.

Specifications of Rat Deoxycholic Acid ELISA kit

Product Information

Cat. No.

E02D0314

Product Name

Rat Deoxycholic Acid ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 mg/ml

Sensitivity

0.1 mg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/mL

1 vial

STANDARD B (0.5mL)

0.5 mg/mL

1 vial

STANDARD C (0.5mL)

1.0 mg/mL

1 vial

STANDARD D (0.5mL)

2.5 mg/mL

1 vial

STANDARD E (0.5mL)

5.0 mg/mL

1 vial

STANDARD F (0.5mL)

10 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DCA ELISA kit uses an anti-DCA antibody and an DCA-HRP conjugate in a competitive enzyme immunoassay method. DCA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DCA antibody binding site between DCA from samples and DCA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DCA. Since the number of sites is limited, as more sites are occupied by DCA from the sample, fewer sites are left to bind DCA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Deoxycholic Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DCA and analogues was observed.


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Summary of the Assay Procedure for Rat Deoxycholic Acid ELISA kit

Summary of the Assay Procedure for Rat Deoxycholic Acid ELISA kit

Citations of Rat Deoxycholic Acid ELISA kit

E02D0314 has been referenced in the below publications:

Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.

Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.

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