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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Glutathione peroxidase ELISA kit

E02G0369 Rat Glutathione peroxidase ELISA kit

The Rat Glutathione peroxidase ELISA kit can be used to identify samples from the rat species. Glutathione peroxidase can also be called GPx, GSHPx.

Specifications of Rat Glutathione peroxidase ELISA kit

Product Information

Cat. No.

E02G0369

Product Name

Rat Glutathione peroxidase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GSH Px ELISA kit uses an anti-GSH Px antibody and an GSH Px-HRP conjugate in a competitive enzyme immunoassay method. GSH Px-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSH Px antibody binding site between GSH Px from samples and GSH Px-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSH Px. Since the number of sites is limited, as more sites are occupied by GSH Px from the sample, fewer sites are left to bind GSH Px-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH Px concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Glutathione peroxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GSH Px and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Glutathione peroxidase ELISA kit

Summary of the Assay Procedure for Rat Glutathione peroxidase ELISA kit

Citations of Rat Glutathione peroxidase ELISA kit

E02G0369 has been referenced in the below publications:

Beneficial Effects of Lactobacillus plantarum Strains on Non-Alcoholic Fatty Liver Disease in High Fat/High Fructose Diet-Fed Rats.

Gastroprotective Effects of Fermented Lotus Root against Ethanol/HCl-Induced Gastric Mucosal Acute Toxicity in Rats.

Beneficial Effects of Lactobacillus plantarum Strains on Non-Alcoholic Fatty Liver Disease in High Fat/High Fructose Diet-Fed Rats.

Rosmarinus officinalis L.hexane extract: phytochemical analysis, nanoencapsulation, and in silico, in vitro, and in vivo anti‑photoaging potential evaluation.

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