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BlueGene Biotech Rat P 38 Mitogen activated Protein Kinase ELISA kit

E02P0038 Rat P 38 Mitogen activated Protein Kinase ELISA kit

The Rat P 38 Mitogen activated Protein Kinase ELISA kit can be used to identify samples from the rat species. P 38 Mitogen activated Protein Kinase can also be called P38MAPK.

Specifications of Rat P 38 Mitogen activated Protein Kinase ELISA kit

Product Information

Cat. No.

E02P0038

Product Name

Rat P 38 Mitogen activated Protein Kinase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

P38MAPK ELISA kit uses an anti-P38MAPK antibody and an P38MAPK-HRP conjugate in a competitive enzyme immunoassay method. P38MAPK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P38MAPK antibody binding site between P38MAPK from samples and P38MAPK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P38MAPK. Since the number of sites is limited, as more sites are occupied by P38MAPK from the sample, fewer sites are left to bind P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P38MAPK concentration in each sample is interpolated from this standard curve.


Quality Control on Rat P 38 Mitogen activated Protein Kinase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between P38MAPK and analogues was observed.


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Summary of the Assay Procedure for Rat P 38 Mitogen activated Protein Kinase ELISA kit

Summary of the Assay Procedure for Rat P 38 Mitogen activated Protein Kinase ELISA kit

Citations of Rat P 38 Mitogen activated Protein Kinase ELISA kit

E02P0038 has been referenced in the below publications:

The Role of Resolvin D1 on acute reflux esophagitis with DHA intervention in Rats.


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