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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Sex Determining Region Y Box Protein 9 ELISA kit

  • Neurobiology
  • Epigenetics and Nuclear Signaling
  • Developmental Biology
  • Stem Cells

E02S0149 Rat Sex Determining Region Y Box Protein 9 ELISA kit

The Rat Sex Determining Region Y Box Protein 9 ELISA kit can be used to identify samples from the rat species. Sex Determining Region Y Box Protein 9 can also be called CMD1, CMPD1, SRA1, SRXX2, SRXY10, SRY-box 9, SRY-box transcription factor 9, SOX9.

Specifications of Rat Sex Determining Region Y Box Protein 9 ELISA kit

Product Information

Cat. No.

E02S0149

Product Name

Rat Sex Determining Region Y Box Protein 9 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SOX9 ELISA kit uses an anti-SOX9 antibody and an SOX9-HRP conjugate in a competitive enzyme immunoassay method. SOX9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SOX9 antibody binding site between SOX9 from samples and SOX9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SOX9. Since the number of sites is limited, as more sites are occupied by SOX9 from the sample, fewer sites are left to bind SOX9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOX9 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Sex Determining Region Y Box Protein 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SOX9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Sex Determining Region Y Box Protein 9 ELISA kit

Summary of the Assay Procedure for Rat Sex Determining Region Y Box Protein 9 ELISA kit

Citations of Rat Sex Determining Region Y Box Protein 9 ELISA kit

E02S0149 has been referenced in the below publications:

Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.


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