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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Triglyceride ELISA kit

E02T0018 Rat Triglyceride ELISA kit

The Rat Triglyceride ELISA kit can be used to identify samples from the rat species. Triglyceride can also be called TAG, Triacylglycerol, Triacylglyceride, TG.

Specifications of Rat Triglyceride ELISA kit

Product Information

Cat. No.

E02T0018

Product Name

Rat Triglyceride ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 mg/dL

Sensitivity

1.0 mg/dl

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/dL

1 vial

STANDARD B (0.5mL)

5.0 mg/dL

1 vial

STANDARD C (0.5mL)

10 mg/dL

1 vial

STANDARD D (0.5mL)

25 mg/dL

1 vial

STANDARD E (0.5mL)

50 mg/dL

1 vial

STANDARD F (0.5mL)

100 mg/dL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TG ELISA kit uses an anti-TG antibody and an TG-HRP conjugate in a competitive enzyme immunoassay method. TG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TG antibody binding site between TG from samples and TG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TG. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Triglyceride ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Triglyceride ELISA kit

Summary of the Assay Procedure for Rat Triglyceride ELISA kit

Citations of Rat Triglyceride ELISA kit

E02T0018 has been referenced in the below publications:

Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.

Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.

Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.

Study on Antioxidation and Reducing Blood Lipid of Chilli Seed Oil.

A high-fructose diet in rats induces systemic iron deficiency and hepatic iron overload by an inflammation mechanism.


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