E02M0352 Rat Mucin 2 ELISA kit
The Rat Mucin 2 ELISA kit can be used to identify samples from the rat species. Mucin 2 can also be called MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal, MUC2.
Specifications of Rat Mucin 2 ELISA kit
Product Information | |
Cat. No. | E02M0352 |
Product Name | Rat Mucin 2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MUC2 ELISA kit uses an anti-MUC2 antibody and an MUC2-HRP conjugate in a competitive enzyme immunoassay method. MUC2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MUC2 antibody binding site between MUC2 from samples and MUC2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MUC2. Since the number of sites is limited, as more sites are occupied by MUC2 from the sample, fewer sites are left to bind MUC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Mucin 2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MUC2 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Mucin 2 ELISA kit
Citations of Rat Mucin 2 ELISA kit
E02M0352 has been referenced in the below publications：
Protective Effects and Mechanism of Erythropoietin on Necrotizing Enterocolitis in a Neonatal Rat Model.
