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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Thromboxanes B2 ELISA kit

E02T0025 Rat Thromboxanes B2 ELISA kit

The Rat Thromboxanes B2 ELISA kit can be used to identify samples from the rat species. Thromboxanes B2 can also be called TXB2, hromboxane-b, Thromboxane B2, TXB 2.

Specifications of Rat Thromboxanes B2 ELISA kit

Product Information

Cat. No.

E02T0025

Product Name

Rat Thromboxanes B2 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TXB2 ELISA kit uses an anti-TXB2 antibody and an TXB2-HRP conjugate in a competitive enzyme immunoassay method. TXB2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TXB2 antibody binding site between TXB2 from samples and TXB2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TXB2. Since the number of sites is limited, as more sites are occupied by TXB2 from the sample, fewer sites are left to bind TXB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXB2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Thromboxanes B2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TXB2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Thromboxanes B2 ELISA kit

Summary of the Assay Procedure for Rat Thromboxanes B2 ELISA kit

Citations of Rat Thromboxanes B2 ELISA kit

E02T0025 has been referenced in the below publications:

Influence of angelica essential oil aromatherapy on rats model of dysmenorrhea.

Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.

Experimental animal model to study iron overload and iron chelation and review of other such models.


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