E02U0025 Rat Urine protein ELISA
The Rat Urine protein ELISA can be used to identify samples from the rat species. Urine protein can also be called UP
Specifications of Rat Urine protein ELISA
Product Information | |
Cat. No. | E02U0025 |
Product Name | Rat Urine protein ELISA |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 mg/ml |
Sensitivity | 0.1 mg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 mg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 mg/mL | 1 vial |
STANDARD F (0.5mL) | 10 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
UP ELISA kit uses an anti-UP antibody and an UP-HRP conjugate in a competitive enzyme immunoassay method. UP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-UP antibody binding site between UP from samples and UP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of UP. Since the number of sites is limited, as more sites are occupied by UP from the sample, fewer sites are left to bind UP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Urine protein ELISA
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between UP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Urine protein ELISA
Citations of Rat Urine protein ELISA
E02U0025 has been referenced in the below publications：
Effect of Huayu Tongluo Traditional Chinese Medicine on Skeleton Protein in Podocytes of Rats with Diabetic Nephropathy.Effect of Combined Prescription and Separate Prescription of Huayu Tongluo Traditional Chinese Medicine on Blood Correlation Index and the Expression of ZO-1 in Ｒenal Podocytes of DN Ｒats.
