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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Immunoglobulin A ELISA kit (E14I0021)

E14I0021 Sheep Immunoglobulin A ELISA kit

Sheep Immunoglobulin A ELISA kit is suitable for the detection of samples from sheep species. Immunoglobulin A can also be called Ig A, Immunoglobulin A, IgA.

Products

Specifications of Sheep Immunoglobulin A ELISA kit

Product Information

Cat. No.

E14I0021

Product Name

Sheep Immunoglobulin A ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 mg/mL

Sensitivity

0.1 mg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 mg/mL

1 vial

STANDARD C (0.5mL)

5 mg/mL

1 vial

STANDARD D (0.5mL)

10 mg/mL

1 vial

STANDARD E (0.5mL)

25 mg/mL

1 vial

STANDARD F (0.5mL)

50 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IgA. Standards or samples are then added to the microtiter plate wells and IgA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgA are added to each well to “sandwich” the IgA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IgA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Immunoglobulin A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Immunoglobulin A ELISA kit

Summary of the Assay Procedure for Sheep Immunoglobulin A ELISA kit

Citations of Sheep Immunoglobulin A ELISA kit

E14I0021 has been referenced in the below publications:

Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.

Effects of α-Lipoic Acid on Growth Performance, Antioxidant and Immunity of Sheep under Heat Stress.

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