E14I0345 Sheep Interferon γ ELISA kit
Sheep Interferon γ ELISA kit is suitable for the detection of samples from sheep species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.
Sheep Interferon γ ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
IFNγ ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNγ antibody and an IFNγ-HRP conjugate. The assay sample and buffer are incubated together with IFNγ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNγ concentration since IFNγ from samples and IFNγ-HRP conjugate compete for the anti-IFNγ antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between IFNγ and analogues was observed.
E14I0345 has been referenced in the below publications:
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.
The Comparison of Some Blood Immune Parameters in Sheep.
Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
Identifcations of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.
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