E11E0023 Bovine estradiol ELISA kit
The Bovine estradiol ELISA kit can be used to identify samples from the bovine species. estradiol can also be called Estradiol, E2 estradiol, 17B-Estradiol, E2, Oestradiol, Beta-Estradiol.
Specifications of Bovine estradiol ELISA kit
Product Information | |
Cat. No. | E11E0023 |
Product Name | Bovine estradiol ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
E2 ELISA kit uses an anti-E2 antibody and an E2-HRP conjugate in a competitive enzyme immunoassay method. E2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-E2 antibody binding site between E2 from samples and E2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of E2. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine estradiol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between E2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine estradiol ELISA kit
Citations of Bovine estradiol ELISA kit
E11E0023 has been referenced in the below publications：
impact of fertility-promoting powder on trace elements and hormone in serum of dairy cattle with ovarian static disease.
A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle.
study on the impact of fertility-promoting powder on reproductive hormones in serum of dairy cattle with persistent corpus luteum
Study on Effect of Reproductive Performance of Cows on Biotin and Endocrine Mechanism.
Study on the Dagnosis Ovarian Disease by B-mode Ultrasound Scanner and Optimization of the Treatment Protocol on Different Stage of Postpartum in Dairy Cows.
The Study on the Therapy Options of Uterine Involution in Postpartum Cows.
中药复方理囊散对奶牛子宫复旧期间生殖激素的影响。
The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility-promoting powder.
Effects of Vitamin D on Follicular Granulose Cells of Cattle Ovarian.
奶牛卵巢囊肿的综合诊断。
