Bovine Carnitine palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called as arnitine acyltransferase I; CPTI; CAT1; CoA:carnitine acyl transferase (CCAT); or palmitoylCoA transferase I.
Specifications Of E11C0055 Bovine CPT 1 ELISA Kit
Product's Information | |
Cat. NO. | E11C0055 |
Product Name | Bovine Carnitine palmitoyltransferase I ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated. |
Quality Control On Bovine CPT 1 ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 87-105 | |
Linearity | Diluent ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between CPT 1 and analogues was observed. | |
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Summary Of The Assay Procedures For E11C0055 Bovine CPT 1 ELISA Kit
Citations Of Bovine Carnitine Palmitoyltransferase I ELISA Kit
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