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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Follicle Stimulating Hormone ELISA kit (E11F0001)

E11F0001 Bovine Follicle Stimulating Hormone ELISA kit

The Bovine Follicle Stimulating Hormone ELISA kit can be used to identify samples from the bovine species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta.

Products

Specifications of Bovine Follicle Stimulating Hormone ELISA kit

Product Information

Cat. No.

E11F0001

Product Name

Bovine Follicle Stimulating Hormone ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FSH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FSH. Standards or samples are then added to the microtiter plate wells and FSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FSH are added to each well to “sandwich” the FSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Follicle Stimulating Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FSH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Follicle Stimulating Hormone ELISA kit

Summary of the Assay Procedure for Bovine Follicle Stimulating Hormone ELISA kit

Citations of Bovine Follicle Stimulating Hormone ELISA kit

E11F0001 has been referenced in the below publications:

impact of fertility-promoting powder on trace elements and hormone in serum of dairy cattle with ovarian static disease.

A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle.

The study of monitoring and diagnostic methods of metritis in dairy cows at different postparturient stages.

Study on the Dagnosis Ovarian Disease by B-mode Ultrasound Scanner and Optimization of the Treatment Protocol on Different Stage of Postpartum in Dairy Cows.

The Study on the Therapy Options of Uterine Involution in Postpartum Cows.

中药复方理囊散对奶牛子宫复旧期间生殖激素的影响。

The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility-promoting powder.

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertility-promoting Power in Dairy Cows.

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