The Bovine Albumin ELISA kit can be used to identify samples from the bovine species. Albumin can also be called ALB; Serum albumin.
Specifications Of E11A0028 Bovine ALB ELISA Kit
Product's Information | |
Cat NO. | E11A0028 |
Product Name | Bovine Albumin ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFIALBION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 10 ug/mL | 1 vial |
STANDARD E (0.5mL) | 25 ug/mL | 1 vial |
STANDARD F (0.5mL) | 50 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALB ELISA kit uses an anti-ALB antibody and an ALB-HRP conjugate in a competitive enzyme immunoassay method. ALB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ALB antibody binding site between ALB from samples and ALB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ALB. Since the number of sites is limited, as more sites are occupied by ALB from the sample, fewer sites are left to bind ALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALB concentration in each sample is interpolated from this standard curve. |
Quality Control On Bovine ALB ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 95-102 | |
Linearity | Diluent ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ALB and analogues was observed. | |
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Summary Of The Assay Procedures For E11A0028 Bovine ALB ELISA Kit
Citations Of Bovine Albumin ELISA Kit
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows
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