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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Insulin like Growth Factor1 ELISA kit (E11I0009)

E11I0009 Bovine Insulin like Growth Factor1 ELISA kit

The Bovine Insulin like Growth Factor1 ELISA kit can be used to identify samples from the bovine species. Insulin like Growth Factor1 can also be called IBP1, IGF IA, IGF IB, IGFI, IGFIA, Insulin Like Growth Factor 1, Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, MGF, Mechano growth factor, MGF, Somatomedia C, Somatomedin C.

Products

Specifications of Bovine Insulin like Growth Factor1 ELISA kit

Product Information

Cat. No.

E11I0009

Product Name

Bovine Insulin like Growth Factor1 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGF1 ELISA kit uses an anti-IGF1 antibody and an IGF1-HRP conjugate in a competitive enzyme immunoassay method. IGF1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IGF1 antibody binding site between IGF1 from samples and IGF1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IGF1. Since the number of sites is limited, as more sites are occupied by IGF1 from the sample, fewer sites are left to bind IGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Insulin like Growth Factor1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGF1 and analogues was observed.


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Summary of the Assay Procedure for Bovine Insulin like Growth Factor1 ELISA kit

Summary of the Assay Procedure for Bovine Insulin like Growth Factor1 ELISA kit

Citations of Bovine Insulin like Growth Factor1 ELISA kit

E11I0009 has been referenced in the below publications:

Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro.

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