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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Insulin ELISA kit

E11I0004 Bovine Insulin ELISA kit

The Bovine Insulin ELISA kit can be used to identify samples from the bovine species. Insulin can also be called ILPR,Insulin A chain, Insulin B chain,IRDN, INS.

Specifications of Bovine Insulin ELISA kit

Product Information

Cat. No.

E11A0028

Product Name

Bovine Albumin ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

INS ELISA kit uses an anti-INS antibody and an INS-HRP conjugate in a competitive enzyme immunoassay method. INS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INS antibody binding site between INS from samples and INS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INS. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Insulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between INS and analogues was observed.


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Summary of the Assay Procedure for Bovine Insulin ELISA kit

Summary of the Assay Procedure for Bovine Insulin ELISA kit

Citations of Bovine Insulin ELISA kit

E11I0004 has been referenced in the below publications:

Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.

Supplementation with fat-coated rumen-protected glucose during the transition period enhances milk production and influences blood biochemical parameters of liver function and inflammation in dairy cows.

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