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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Interleukin 12 ELISA kit

E11I0033 Bovine Interleukin 12 ELISA kit

The Bovine Interleukin 12 ELISA kit can be used to identify samples from the bovine species. Interleukin 12 can also be called IL12, CLMF p35, CLMF1, CTL maturation factor (TcMF), Cytotoxic lymphocyte maturation factor 1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL 12 subunit p35, IL12A, Interleukin 12 alpha chain, Interleukin 12 p35, Interleukin 12A, Natural killer cell stimulatory factor 1, CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12 subunit p35, IL12A, IL12A_HUMAN, Interleukin 12 p35, Interleukin 12A, Interleukin-12 subunit alpha, NFSK, NK cell stimulatory factor chain 1, NKSF1. NK cell stimulatory factor chain 1.

Specifications of Bovine Interleukin 12 ELISA kit

Product Information

Cat. No.

E11I0033

Product Name

Bovine Interleukin 12 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL 12. Standards or samples are then added to the microtiter plate wells and IL 12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL 12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL 12 are added to each well to “sandwich” the IL 12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL 12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 12 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Interleukin 12 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 12 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Interleukin 12 ELISA kit

Summary of the Assay Procedure for Bovine Interleukin 12 ELISA kit

Citations of Bovine Interleukin 12 ELISA kit

E11I0033 has been referenced in the below publications:

Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

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