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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Interleukin 6 ELISA kit (E11I0006)

E11I0006 Bovine Interleukin 6 ELISA kit

The Bovine Interleukin 6 ELISA kit can be used to identify samples from the bovine species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL6, IL-6, IL 6.

Products

Specifications of Bovine Interleukin 6 ELISA kit

Product Information

Cat. No.

E11I0006

Product Name

Bovine Interleukin 6 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL6 ELISA kit uses an anti-IL6 antibody and an IL6-HRP conjugate in a competitive enzyme immunoassay method. IL6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL6 antibody binding site between IL6 from samples and IL6-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL6. Since the number of sites is limited, as more sites are occupied by IL6 from the sample, fewer sites are left to bind IL6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL6 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Interleukin 6 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL6 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Interleukin 6 ELISA kit

Summary of the Assay Procedure for Bovine Interleukin 6 ELISA kit

Citations of Bovine Interleukin 6 ELISA kit

E11I0006 has been referenced in the below publications:

Effects of Fertility-Promoting Powder on Trace Elements and Interleukin in Serum in Infertile Dairy Cattle.

To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care.

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertilitypromoting Power in Dairy Cows.

蒲公英水提物对断奶仔猪免疫功能和血清抗氧化功能的效应试验。

SREBP-1c overactivates ROS-mediated hepatic NF-κB inflammatory pathway in dairy cows with fatty liver.



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