E11M0005 Bovine Melatonin ELISA kit
The Bovine Melatonin ELISA kit can be used to identify samples from the bovine species. Melatonin can also be called MLT.
Bovine Melatonin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
MLT ELISA kit uses an anti-MLT antibody and an MLT-HRP conjugate in a competitive enzyme immunoassay method. MLT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MLT antibody binding site between MLT from samples and MLT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MLT. Since the number of sites is limited, as more sites are occupied by MLT from the sample, fewer sites are left to bind MLT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between MLT and analogues was observed.
E11M0005 has been referenced in the below publications：
Effect of exposure to extremely low frequency magnetic fields on melatonin levels in calves is seasonally dependent.
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