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BlueGene Biotech Human Luteinizing Hormone ELISA kit (E01L0021)

E01L0021 Human Luteinizing Hormone ELISA kit

The Human Luteinizing Hormone ELISA kit can be used to identify samples from the human species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormone.

Products

Specifications of Human Luteinizing Hormone ELISA kit

Product Information

Cat. No.

E01L0021

Product Name

Human Luteinizing Hormone ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 mIU/mL

Sensitivity

1.0 mIU/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

mIU/mL

1 vial

STANDARD B (0.5mL)

10 mIU/mL

1 vial

STANDARD C (0.5mL)

25 mIU/mL

1 vial

STANDARD D (0.5mL)

50 mIU/mL

1 vial

STANDARD E (0.5mL)

100 mIU/mL

1 vial

STANDARD F (0.5mL)

250 mIU/mL1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LH ELISA kit uses an anti-LH antibody and an LH-HRP conjugate in a competitive enzyme immunoassay method. LH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LH antibody binding site between LH from samples and LH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LH. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.


Quality Control on Human Luteinizing Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Luteinizing Hormone ELISA kit

Summary of the Assay Procedure for Human Luteinizing Hormone ELISA kit

Citations of Human Luteinizing Hormone ELISA kit

E01L0021 has been not referenced.


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