E11T0018 Bovine Triglyceride ELISA kit
The Bovine Triglyceride ELISA kit can be used to identify samples from the bovine species. Triglyceride can also be called TAG, Triacylglycerol, Triacylglyceride, TG.
Bovine Triglyceride ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
TG ELISA kit uses an anti-TG antibody and an TG-HRP conjugate in a competitive enzyme immunoassay method. TG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TG antibody binding site between TG from samples and TG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TG. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between TG and analogues was observed.
E11T0018 has been referenced in the below publications：
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.
Related Bluegene Biotech Products
BlueGene Biotech News