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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Alkaline Phosphatase ELISA kit (E11A0031)

E11A0031 Bovine Alkaline Phosphatase ELISA kit

For the detection of samples from bovine species, use an ELISA kit for bovine alkaline phosphatase. Alkaline Phosphatase can also be called AKP, ALKP, Basic Phosphatase.

Products

Specifications of Bovine Alkaline Phosphatase ELISA kit

Product Information

Cat. No.

E11A0031

Product Name

Bovine Alkaline Phosphatase ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ALP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALP antibody and an ALP-HRP conjugate. The assay sample and buffer are incubated together with ALP-HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for the HRP enzyme. The product of the enzyme-substrate reaction forms a blue-colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of the color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALP concentration since ALP from samples and ALP-HRP conjugate compete for the anti-ALP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALP from the sample, fewer sites are left to bind ALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALP concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Alkaline Phosphatase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-103%

Linearity

Diluent Ratio

Range %

1:2

90-105

1:4

88-108

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ALP and analogues was observed.


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Summary of the Assay Procedure for Bovine Alkaline Phosphatase ELISA kit

Summary of the Assay Procedure for Bovine Alkaline Phosphatase ELISA kit

Citations of Bovine Alkaline Phosphatase ELISA Kit

E11A0031 has been referenced in the below publications:

Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.

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