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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit (E12S0258)

E12S0258 Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit is suitable for the detection of samples from Chicken species. Soluble Intercellular Adhesion Molecule 1 can also be called SICAM1, SBB2, SCD54, SP3.58, sintercellular adhesion molecule 1.

Products

Specifications of Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Product Information

Cat. No.

E12S0258

Product Name

Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SICAM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SICAM1 antibody and an SICAM1-HRP conjugate. The assay sample and buffer are incubated together with SICAM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SICAM1 concentration since SICAM1 from samples and SICAM1-HRP conjugate compete for the anti-SICAM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SICAM1 from the sample, fewer sites are left to bind SICAM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SICAM1 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SICAM1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Summary of the Assay Procedure for Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

Citations of Chicken Soluble Intercellular Adhesion Molecule 1 ELISA kit

E12S0258 has been referenced in the below publications:

EFFECT OF CLINOPTILOLITE ON GUT PROTECTION OF BROILERS AND STUDY OF RELEVANT MECHANISM.


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