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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Cyclic Guanosine Monophosphate ELISA kit (E12G0003)

E12G0003 Chicken Cyclic Guanosine Monophosphate ELISA kit

Chicken Cyclic Guanosine Monophosphate ELISA kit is suitable for the detection of samples from chicken species. Cyclic Guanosine Monophosphate can also be called Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate, cGMP.

Products

Specifications of Chicken Cyclic Guanosine Monophosphate ELISA kit

Product Information

Cat. No.

E12G0003

Product Name

Chicken Cyclic Guanosine Monophosphate ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cGMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cGMP antibody and an cGMP-HRP conjugate. The assay sample and buffer are incubated together with cGMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between cGMP from samples and cGMP-HRP conjugate for the anti-cGMP antibody binding site, the intensity of the color is inversely correlated with the concentration of cGMP. As more sites are taken up by cGMP from the sample, fewer sites are left that can bind cGMP-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.


Quality Control on Chicken Cyclic Guanosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cGMP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Cyclic Guanosine Monophosphate ELISA kit

Summary of the Assay Procedure for Chicken Cyclic Guanosine Monophosphate ELISA kit

Citations of Chicken Cyclic Guanosine Monophosphate ELISA kit

E12G0003 has been referenced in the below publications:

Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.


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