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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit (E12T0023)

E12T0023 Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit is suitable for the detection of samples from chicken species. Tumor necrosis factor related apoptosis inducing ligand can also be called TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A, tumor necrosis factor superfamily member 10, TNF superfamily member 10.

Products

Specifications of Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Product Information

Cat. No.

E12T0023

Product Name

Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

0.5 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

TRAIL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TRAIL antibody and an TRAIL-HRP conjugate. The assay sample and buffer are incubated together with TRAIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRAIL concentration since TRAIL from samples and TRAIL-HRP conjugate compete for the anti-TRAIL antibody binding site. Since the number of sites is limited, as more sites are occupied by TRAIL from the sample, fewer sites are left to bind TRAIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRAIL concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

88-116.69%

Linearity

Diluent Ratio

Range %

1:2

95-117

1:4

91-107

1:8

89 -104

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TRAIL and analogues was observed.


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Summary of the Assay Procedure for Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Summary of the Assay Procedure for Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

Citations of Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit

E12T0023 has been referenced in the below publications:

Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.

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