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E03A0043 Mouse Adenosine Monophosphate Activated Protein ELISA Kit

The Mouse Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from mouse species. Adenosine Monophosphate Activated Protein can also be called AMPK; AMP-activated protein kinase; 5' AMP-activated protein kinase; 5' adenosine monophosphate-activated protein kinase.

Specifications Of E03A0043 Mouse AMPK ELISA Kit

Porduct's Information

Cat.NO.

E03A0043

Porduct's Name

Mouse Adenosine Monophosphate Activated Protein ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMPK antibody and an AMPK-HRP conjugate. The assay sample and buffer are incubated together with AMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPK concentration since AMPK from samples and AMPK-HRP conjugate compete for the anti-AMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPK from the sample, fewer sites are left to bind AMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve.


Quality Control On Mouse AMPK ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

97-104

Linearity

Diluent ratio

Range %

1:2

96-108

1:4

93-106

1:8

91-103

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between AMPK and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E03A0043 Mouse AMPK ELISA Kit

Summary Of The Assay Procedures For E03A0043 Mouse AMPK ELISA Kit

Citations Of Mouse Adenosine Monophosphate Activated Protein ELISA Kit

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