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E03A0202 Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA Kit

  • Signal Transduction
  • Stem Cells
  • Developmental Biology

Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA kit is suitable for the detection of samples from mouse species. Mullerian Inhibiting Substance/Anti Mullerian Hormone can also be called as MIS/AMH; MIS_HUMAN; Anti muellerian hormone; MIF; Muellerian inhibiting factor; Mullerian inhibiting substance;  Anti-Muellerian hormone.

Specifications Of E03A0202 Mouse MIS/AMH ELISA Kit

Porduct's Information

Cat.NO.

E03A0202

Porduct's Name

Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MIS/AMH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MIS/AMH antibody and an MIS/AMH-HRP conjugate. The assay sample and buffer are incubated together with MIS/AMH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIS/AMH concentration since MIS/AMH from samples and MIS/AMH-HRP conjugate compete for the anti-MIS/AMH antibody binding site. Since the number of sites is limited, as more sites are occupied by MIS/AMH from the sample, fewer sites are left to bind MIS/AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIS/AMH concentration in each sample is interpolated from this standard curve.


Quality Control On Mouse MIS/AMH ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

90-109

Linearity

Diluent ratio

Range %

1:2

88-111

1:4

91-105

1:8

93-107

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between MIS/AMH and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E03A0202 Mouse MIS/AMH ELISA Kit

Summary Of The Assay Procedures For E03A0202 Mouse MIS/AMH ELISA Kit

Citations Of Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA Kit

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