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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Estradiol ELISA kit

E07E0023 Porcine Estradiol ELISA kit

Porcine Estradiol ELISA kit is suitable for detecting samples from porcine species. Estradiol can also be called Estradiol, E2 estradiol, 17B-Estradiol, E2, Oestradiol, or Beta-Estradiol.

Specifications of Porcine Estradiol ELISA kit

Product Information

Cat. No.

E07E0023

Product Name

Porcine Estradiol ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Estradiol ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-107%

Linearity

Diluent Ratio

Range %

1:2

94-110

1:4

89-108

1:8

91-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between E2 and analogues was observed.



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Summary of the Assay Procedure for Porcine Estradiol ELISA kit

Summary of the Assay Procedure for Porcine Estradiol ELISA kit

Citations of Porcine Estradiol ELISA kit

E07E0023 has been referenced in the below publications:

Effects of Insulin and FSH on EProduction of PigOvarian Follicular Granulosa Cells in vitro Culture.

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