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BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit

E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit

Canine Brain Derived Neurotrophic Factor ELISA kit is suitable for the detection of samples from Canine species. Brain Derived Neurotrophic Factor can also be called Neurotrophin, Abrineurin, BDNF.

Specifications of Canine Brain Derived Neurotrophic Factor ELISA kit

Product Information

Cat. No.

E08B0029

Product Name

Canine Brain Derived Neurotrophic Factor ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BDNF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BDNF. Standards or samples are then added to the microtiter plate wells and BDNF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BDNF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BDNF are added to each well to “sandwich” the BDNF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BDNF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Brain Derived Neurotrophic Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-103%

Linearity

Diluent Ratio

Range %

1:2

88-105

1:4

86-105

1:8

83-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BDNF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine Brain Derived Neurotrophic Factor ELISA kit

Summary of the Assay Procedure for Canine Brain Derived Neurotrophic Factor ELISA kit

Citations of Canine Brain Derived Neurotrophic Factor ELISA kit

E08B0029 has been referenced in the below publications:

Effects of Electroacupuncture on Plasma Concentrations of Three Related Neurotrophic Factors in Normal Dogs.

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