Bovine Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from Bovine species. Adenosine Monophosphate Activated Protein can also be called as PRKAa1; AMPKa1; PKA; ACACA kinase.
Specifications Of E11A0043 Bovine AMPK ELISA Kit
Product's Information | |
Cat. NO. | E11A0043 |
Product Name | Bovine Adenosine Monophosphate Activated Protein ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFIAMPKION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMPK antibody and an AMPK-HRP conjugate. The assay sample and buffer are incubated together with AMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPK concentration since AMPK from samples and AMPK-HRP conjugate compete for the anti-AMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPK from the sample, fewer sites are left to bind AMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve. |
Quality Control On Bovine AMPK ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 87-101 | |
Linearity | Diluent ratio | Range % |
1:2 | 85-103 | |
1:4 | 84-108 | |
1:8 | 82-107 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between AMPK and analogues was observed. | |
Summary Of The Assay Procedures For E11A0043 Bovine AMPK ELISA Kit
Citations Of Bovine Adenosine Monophosphate Activated Protein ELISA Kit
β-Hydroxybutyric Sodium Salt Inhibition of Growth Hormone and Prolactin Secretion via the cAMP/PKA/CREB and AMPK Signaling Pathways in Dairy Cow Anterior Pituitary Cells
Product: E11A0043 Bovine Adenosine Monophosphate Activated Protein ELISA kit;
