E11C0659 Bovine Complement Fragment 4A ELISA kit
Bovine Complement fragment 4a ELISA kit is suitable for the detection of samples from Bovine species. Complement fragment 4a can also be called C4a anaphylatoxin.
Specifications of Bovine Complement Fragment 4A ELISA kit
Product Information | |
Cat. No. | E11C0659 |
Product Name | Bovine Complement fragment 4a ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
C4a ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-C4a antibody and an C4a-HRP conjugate. The assay sample and buffer are incubated together with C4a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4a concentration since C4a from samples and C4a-HRP conjugate compete for the anti-C4a antibody binding site. Since the number of sites is limited, as more sites are occupied by C4a from the sample, fewer sites are left to bind C4a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4a concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Complement Fragment 4A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 94-103% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 93-108 | |
1:8 | 89-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between C4a and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Complement Fragment 4A ELISA kit
Citations of Bovine Complement Fragment 4A ELISA kit
E11C0659 has been referenced in the below publications:
Three novel single-nucleotide polymorphisms of complement component 4 gene in Chinese Holstein cattle and their associations with milk performance traits and CH50.
