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BlueGene Biotech Chicken BCL2 Associated X Protein ELISA kit

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E12B0032 Chicken BCL2 Associated X Protein ELISA kit

Chicken BCL2 Associated X Protein ELISA kit is suitable for the detection of samples from chicken species. BCL2 Associated X Protein can also be called BAX, BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4.


Specifications of Chicken BCL2 Associated X Protein ELISA kit

Product Information

Cat. No.

E12B0032

Product Name

Chicken BCL2 Associated X Protein ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BAX ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BAX antibody and a BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken BCL2 Associated X Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

85-115.46%

Linearity

Diluent Ratio

Range %

1:2

97-113

1:4

91-106

1:8

87 -104

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BAX and analogues was observed.


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Summary of the Assay Procedure for Chicken BCL2 Associated X Protein ELISA kit

Summary of the Assay Procedure for Chicken BCL2 Associated X Protein ELISA kit

Citations of Chicken BCL2 Associated X Protein ELISA kit

E12B0032 has been referenced in the below publications:

Experimental study of the inhibition effect of "Phrul sByor Chem Mo": a Tibetan medical prescription, on digestive system tumor cell lines in vitro.

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