Sheep Anti Interleukin 10 ELISA kit is suitable for the detection of samples from sheep species. Anti Interleukin 10 can also be called as Anti IL10; Anti CSIF; Anti GVHDS; Anti IL-10; Anti IL10A; Anti TGIF; Anti interleukin 10.
Specifications Of E14A0081 Sheep Anti IL 10 ELISA Kit
Porduct's Information | |
Cat.NO. | E14A0081 |
Porduct's Name | Sheep Anti Interleukin 10 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Anti-IL 10 ELISA kit applies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve. |
Quality Control On Sheep Anti IL 10 ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 87-118.37 | |
Linearity | Diluent ratio | Range % |
1:2 | 98-117 | |
1:4 | 93-106 | |
1:8 | 91-105 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between Anti IL 10 and analogues was observed. | |
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Summary Of The Assay Procedures For E14A0081 Sheep Anti IL 10 ELISA Kit
Citations Of Sheep Anti Interleukin 10 ELISA Kit
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep
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