E14A0081 Sheep Anti Interleukin 10 ELISA kit
Sheep Anti Interleukin 10 ELISA kit is suitable for the detection of samples from sheep species. Anti Interleukin 10 can also be called Anti IL10, Anti CSIF, Anti GVHDS, Anti IL-10, Anti IL10A, Anti TGIF, Anti interleukin 10, IL10 Ab, IL-10Ab, IL10 Ab.
Product Information | |
Cat. No. | E14A0081 |
Product Name | Sheep Anti Interleukin 10 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Anti-IL 10 ELISA kit applies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-118.37% | |
Linearity | Diluent Ratio | Range % |
1:2 | 98-117 | |
1:4 | 93-106 | |
1:8 | 91-105 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Anti IL 10 and analogues was observed. | |
E14A0081 has been referenced in the below publications:
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.
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