E05I0345 Guinea Pig Interferon γ ELISA kit
Guinea Pig Interferon γ ELISA kit is suitable for the detection of samples from Guinea Pig species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.
Specifications of Guinea Pig Interferon γ ELISA kit
Product Information | |
Cat. NO. | E05I0345 |
Product Name | Guinea Pig Interferon γ ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IFNγ ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNγ antibody and an IFNγ-HRP conjugate. The assay sample and buffer are incubated together with IFNγ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IFNγ antibody binding site between IFNγ from samples and IFNγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IFNγ. As more sites are taken up by IFNγ from the sample, fewer sites are left that can bind IFNγ-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the IFNγ concentration in each sample. |
Quality Control on Guinea Pig Interferon γ ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IFNγ and analogues was observed. | |
BlueGene Biotech Product
Summary of the Assay Procedure for Guinea Pig Interferon γ ELISA kit
Citations of Guinea Pig Interferon γ ELISA kit
E05I0345 has been referenced in the below publications：
High-Level Cellular and Humoral Immune Responses in Guinea Pigs Immunized Intradermally with a Heat-Inactivated Varicella-Zoster Virus Vaccine.
