E05S0009 Guinea Pig Substance P ELISA kit
Guinea Pig Substance P ELISA kit is suitable for the detection of samples from Bovine species. Substance P can also be called SP, S.P.
Specifications of Guinea Pig Substance P ELISA kit
Product Information | |
Cat. NO. | E05S0009 |
Product Name | Guinea Pig Substance P ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
S.P ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-S.P antibody and an S.P-HRP conjugate. The assay sample and buffer are incubated together with S.P-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-S.P antibody binding site between S.P from samples and S.P-HRP conjugate, the intensity of the color is inversely proportional to the concentration of S.P. As more sites are taken up by S.P from the sample, fewer sites are left that can bind S.P-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the S.P concentration in each sample. |
Quality Control on Guinea Pig Substance P ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between S.P and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Guinea Pig Substance P ELISA kit
Citations of Guinea Pig Substance P ELISA kit
E05S0009 has been referenced in the below publications：
Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure.
“从肝论治, 理气调神”针刺法治疗过敏性鼻炎的实验研究。
