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BlueGene Biotech Human anti cyclic citrullinated peptide antibody ELISA Kit

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E01A2058 Human anti cyclic citrullinated peptide antibody ELISA Kit

The Human anti cyclic citrullinated peptide antibody ELISA Kit can be used to identify samples from the human species. anti cyclic citrullinated peptide antibody can also be called cyclic citrullinated peptide Ab,ACPA.

Specifications of Human anti cyclic citrullinated peptide antibody ELISA Kit

Product Information

Cat. No.

E01A2058

Product Name

Human anti cyclic citrullinated peptide antibody ELISA Kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CCP-Ab ELISA kit uses an anti-CCP-Ab antibody and an CCP-Ab-HRP conjugate in a competitive enzyme immunoassay method. CCP-Ab-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CCP-Ab antibody binding site between CCP-Ab from samples and CCP-Ab-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CCP-Ab. Since the number of sites is limited, as more sites are occupied by CCP-Ab from the sample, fewer sites are left to bind CCP-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCP-Ab concentration in each sample is interpolated from this standard curve.


Quality Control on Human anti cyclic citrullinated peptide antibody ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CCP-Ab and analogues was observed.


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Summary of the Assay Procedure for Human anti cyclic citrullinated peptide antibody ELISA Kit

Summary of the Assay Procedure for Human anti cyclic citrullinated peptide antibody ELISA Kit

Citations of Human anti cyclic citrullinated peptide antibody ELISA Kit

E01A2058 has been referenced in the below publications:

SERS-based immunoassay of anti-cyclic citrullinated peptide for early diagnosis of rheumatoid arthritis.

Significance of Anti-Carbamylated Protein Antibody Measurement in the Diagnosis of Rheumatoid Arthritis.


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