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BlueGene Biotech Human C-lara cell secretory protein 10 ELISA kit (E01C2721)

E01C2721 Human C-lara cell secretory protein 10 ELISA kit

The Human C-lara cell secretory protein 10 ELISA kit can be used to identify samples from the Human species. C-lara cell secretory protein 10 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1, uteroglobin, uteroglobin-like antigen, UGL, club-cell secretory protein, Clara-cell 16 kD protein, club-cell-specific 10 kD protein, urine protein 1, polychlorinated biphenyl-binding protein, PCB-BP, club cell phospholipid-binding protein, UG, Uteroglobin, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein, Secretoglobin Family 1A Member 1, Clara-Cell Specific 10-kD Protein, blastokinin.

Products

Specifications of Human C-lara cell secretory protein 10 ELISA kit

Product Information

Cat. No.

E01C2721

Product Name

Human C-lara cell secretory protein 10 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CC-10 ELISA kit uses an anti-CC-10 antibody and an CC-10-HRP conjugate in a competitive enzyme immunoassay method. CC-10-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CC-10 antibody binding site between CC-10 from samples and CC-10-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CC-10. Since the number of sites is limited, as more sites are occupied by CC-10 from the sample, fewer sites are left to bind CC-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CC-10 concentration in each sample is interpolated from this standard curve.


Quality Control on Human C-lara cell secretory protein 10 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CC-10 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human C-lara cell secretory protein 10 ELISA kit

Summary of the Assay Procedure for Human C-lara cell secretory protein 10 ELISA kit

Citations of Human C-lara cell secretory protein 10 ELISA kit

E01C2721 has been referenced in the below publications:

Analysis of CC-10 and TNF-a content in bronchoalveolar lavage fluid from coal worker’s pneumoconiosis patients.


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